Semi-automated determination of urinary 17-Ketogenic steroids
نویسندگان
چکیده
منابع مشابه
Fractionation of 17-ketogenic steroids by solvent partition.
J. TECHNIC was (lescribe(l by Few (1) in 1961 for deterniiiiation of I 7-hydroxycorticosteroids in which borohydride re(luction of steroid 20-keto groups was followed by oxidation of adjacent hydroxyl groups in the 17-20 positions with nletaperio(late. The borohydride also reduced all preformed 17-ketosteroids to the corresponding alcoholswhich do not give the Zimmerman reaction-alici reduced s...
متن کاملThe clinical value of urinary 17-ketogenic steroid determinations.
During recent years increasing importance has been attached to the study of adrenal cortical hormones, and consequently much work has been directed towards utilizing the daily output of these hormones and their metabolites in urine as an index of adrenocortical function. Hydrolysis of the conjugated forms in which the steroids are present in urine is a necessary preliminary to their extraction ...
متن کاملClinical Value of Urinary 17-ketogenic Steroid Determinations
During recent years increasing importance has been attached to the study of adrenal cortical hormones, and consequently much work has been directed towards utilizing the daily output of these hormones and their metabolites in urine as an index of adrenocortical function. Hydrolysis of the conjugated forms in which the steroids are present in urine is a necessary preliminary to their extraction ...
متن کاملOxidation of urinary 17-oxogenic steroids with sodium metaperiodate.
Oxidation of urinary pregnane-17,20-diols (17.oxogenic steroids) by sodium metaperiodate is complete within 10 mm at 55#{176}C if the concentration of sodium metaperiodate at the end of the reaction exceeds 1.5 X 10-SM. A safe excess of sodium metaperiodate is present if assay samples, after incubation for 10 mm at 55#{176}C in 3 X 10-EM ethylene glycol, give a positive test with starch-iodide ...
متن کاملThe analysis of urinary 17-oxo steroids by gradient elution.
The present work was initiated by the observation of Reifenstein, Homburger & Dobriner (1950) that 3a: 1 1l-dihydroxy-5p-androstan-17-one (1 l,B-hydroxyaetiocholanolone), a minor component of the urinary 17-oxo steroids, was significantly associated with neoplastic growth. The re-examination of this problem has led to the development of a micro method for the quantitative separation and determi...
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ژورنال
عنوان ژورنال: Sangyo Igaku
سال: 1970
ISSN: 0047-1879,1881-1302
DOI: 10.1539/joh1959.12.6_276_3